Coding
norB

Part:BBa_K1356005

Designed by: Cameron Sargent   Group: iGEM14_BYU_Provo   (2014-10-08)

Nitric oxide reductase (norB) from Pseudomonas aeruginosa PAO1

This gene codes for one of the nitric oxide reductase subunits (norB) that, in connection with the other subunit (norC), converts nitric oxide (NO) into nitrous oxide (N2O). This conversion is the second step in the denitrification pathway from nitrite (NO2-) to nitrogen gas (N2); the other genes that create this pathway can also be found in the iGEM registry (nirS (BBa_K1356003), norC (BBa_K1356004), and nosZ (BBa_K1356006)). A complete description of the original purpose of using this gene in reducing nitrate concentrations in the effluent of wastewater treatment facilities can be found on the BYU Provo 2014 Project Description page.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1390
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 310
    Illegal NgoMIV site found at 352
    Illegal NgoMIV site found at 1254
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
Parameters
None