Part:BBa_I51019

BioBrick base vector, as synthesized by Codon Devices

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

BBa_I51019 is the sequence of the BioBrick base vector as synthesized by Codon Devices, Inc. in Cambridge, MA. The original design of the BioBrick base vector is available as BBa_I51000. BBa_I51001 is the sequence of the BioBrick base vector as reported by Codon Devices, Inc. although the true sequence is something like to BBa_I51019. The exact sequence of the provided DNA is difficult to determine. The corrected BioBrick base vector that was successfully constructed from BBa_I51019 is BBa_I51020.

Usage and Biology

The exact sequence of the provided DNA is difficult to determine. However, BBa_I51019 was used to successfully construct pSB4A5-I52002, pSB4K5-I52002, pSB4C5-I52002, pSB4T5-I52001, pSB3K5-I52002, pSB3C5-I52001, pSB3T5-I52001.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 1327
  Illegal suffix found in sequence at 1
  Illegal EcoRI site found at 3765
  Illegal XbaI site found at 3780
  Illegal SpeI site found at 2440
  Illegal PstI site found at 2454
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1327
  Illegal EcoRI site found at 3765
  Illegal NheI site found at 177
  Illegal NheI site found at 1126
  Illegal NheI site found at 2615
  Illegal NheI site found at 3564
  Illegal SpeI site found at 2
  Illegal SpeI site found at 2440
  Illegal PstI site found at 16
  Illegal PstI site found at 2454
  Illegal NotI site found at 9
  Illegal NotI site found at 1333
  Illegal NotI site found at 2447
  Illegal NotI site found at 3771
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1327
  Illegal EcoRI site found at 3765
  Illegal BglII site found at 1783
  Illegal BglII site found at 4221
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 1327
  Illegal suffix found in sequence at 2
  Illegal EcoRI site found at 3765
  Illegal XbaI site found at 3780
  Illegal SpeI site found at 2440
  Illegal PstI site found at 2454
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1327
  Illegal EcoRI site found at 3765
  Illegal XbaI site found at 1342
  Illegal XbaI site found at 3780
  Illegal SpeI site found at 2
  Illegal SpeI site found at 2440
  Illegal PstI site found at 16
  Illegal PstI site found at 2454
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1671
  Illegal BsaI site found at 4109