Plasmid

Part:BBa_I51000

Designed by: Reshma Shetty   Group:   (2006-03-13)

BioBrick base vector, as originally designed


BBa_I51000 is the original design of the BioBrick base vector BBa_I51020.

Synthesis of BBa_I51000 was problematic. We encountered two types of issues while trying to construct BBa_I51000. Faulty part design adversely impacted the synthesis process and problems during synthesis ended up compromising the design of our parts. Our synthesis difficulties largely stemmed from the dependence of commercial DNA synthesis processes on cloning, assembly and propagation of synthesized DNA in E. coli.

The BioBrick base vector that was successfully constructed is BBa_I51020.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1327
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1327
    Illegal NheI site found at 177
    Illegal NheI site found at 1126
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 1333
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1327
    Illegal BglII site found at 1791
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1327
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1327
    Illegal XbaI site found at 1342
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid/construction
//plasmidbackbone/version/5
Parameters
None