Part:BBa_I51001

BioBrick base vector, as revised by Codon Devices

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

BBa_I51001 is the sequence of the BioBrick base vector as reported by Codon Devices, Inc. in Cambridge, MA. The original design of the BioBrick base vector is available as BBa_I51000. The BioBrick base vector that was successfully constructed is BBa_I51020.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 1327
  Illegal suffix found in sequence at 1
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1327
  Illegal NheI site found at 177
  Illegal NheI site found at 1126
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 1333
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1327
  Illegal BglII site found at 1783
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 1327
  Illegal suffix found in sequence at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1327
  Illegal XbaI site found at 1342
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1671