This glossary is always under construction! You can contribute by adding entries or simply linking them to relevant information. If you write a more comprehensive article, please add a short description on this page in addition to the link.
- Abstraction Hierarchy
- A hierarchy in which individuals work independently at each level.
- acyl-homoserine lactones (acyl-HSLs)
- Small signalling molecules
- BioBrick Component
- A portion of a Biobrick device that is available as a BioBrick product. A protein coding region is a BioBrick device if it is available as a BioBrick product and documented in the BioBrick Data Book.
- BioBrick Device
- A BioBrick system consisting of one or more BioBrick components that together instantiate a full logical or functional process. Normally, this consists of input protein coding regions that code for the input proteins and a BioBrick computational body that processes the input proteins and generates TIPS at the output sites.
- canonical model
- a simplified model that adequately represents the components and interactions under study (the actual system may or may not be structured like the canonical model).
- cell-to-cell signalling
- communication between an individual cell and its neighbors in culture or on a plate.
- the cell that contains and supports a synthetic biology system
- capable of combining to make a larger whole. In the context of BioBricks, the assembly of two BioBricks creates a new, single BioBrick that can, in turn, be used in downstream assemblies.
- A part which produces proteins in reponse to some stimuli.
- Receives the concentration of repressor A and, via gene expression, outputs the concentration of repressor B.
- LVA Tag
- A tag for degradation.
- An operon for self-regulation production of luminescent proteins.
- A process for isolating small amounts of plasmid DNA from bacteria by lysing the cells and precipitating chromosomal DNA and other cell structures.
- Output High (O_H)
- This is an output characteristic from a transfer function (see "output curve" entry below)
- Output Curve
- The current formula idealizing the output curve for <math>(a*c)/(1 + ((i/b)^n)</math>
- PlateMate Plus
- A robot utilized by iGEM to produce large numbers of identical 384-well distribution plates.
- Polymerase Per Second: A measure of number of times that an RNA Polymerase passes out of the regulatory region and into the protein coding region of a BioBrick.
- Protein Coding Region
- A section of DNA that can cause the generation of a specific protein. In BioBricks, this includes the BioBrick prefix and suffix, the Ribosome Binding Site (RBS), the codons for the protein's amino acids, and the stop codon.
- Protein Generator
- A part which is able to produce proteins in response to some stimuli.
- Quad Part Inverter (QPI)
- QPI refers to a Quad-Part Inverter, that is, a PoPS-based inverter composed of four sub-parts: a ribosome binding site, a coding region for a repressor protein (ie. cI lambda), a Help:Terminators, and the promoter (ie. pLamdba) which is regulated by the coded repressor protein
- Regulatory Region
- The canonical model of a BioBrick component asserts that proteins generated as a result of other logical or cellular processes (see Protein Coding Region) as well as other active molecules interact with the computational body of a BioBrick component to produce the output signal. The genetic code of the computational body is represented by a Regulatory Region of DNA.
- A gene that helps visualize or quantify the output of a device or part.
- Restriction enzymes
- An enzyme that recognizes a particular sequence of bases in DNA, and then cuts the DNA's backbones at that point.
- Ribosome Binding Site
- Small Molecule Sensors
- A small molecule sensor can trigger gene expression when a small molecule becomes present in a cell's environment. Generally, each sensor is specific only to one or a range of small molecules. These sensors are generally surface proteins that can bind to a small molecule ligand. Upon binding, the surfact protein changes conformation, causing one or more intra-cell signals to induce gene expression. A common method of creating a small molecule sensor is to find (discover or literature research) a cellular pathway that is induced by a certain molecule. The surface protein(s) and intial promotors of the mechanism can be isolated from the wild type cellular pathway and made into a BioBrick part. The isolated surface protein + promotor can be put upstream of a new expression gene and inserted into a cell.
- Signalling Molecules
- A molecules involved in (generally) intercellular signalling methods
- Small functional sequences of amino acids.
- Transfer function
- The ratio of output and input in a system.