Device:BBa D0001

Designed by: Francis Crick and Jim Watson

AHL Receiver
Inputs: Small molecule ([3OC6HSL])
Outputs: Transcriptional activity (PoPS)
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Description Subparts
BBa_D0001 is a receiver device that can be used to sense the level of a cell-cell signaling molecule 3OC6HSL. A transcription factor, LuxR (BBa_C0062) that is active in the presence of 3OC6HSL is controlled by a TetR (BBa_C0040) regulated operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. This device is known to work in many E. coli strains at a range of temperatures and in a variety of media types.
tetR
R0040		LuxR
I0462		lux pR
R0062

Performance

Figure 1 - Transfer function of BBa_D0001. The data points represent the mean of 6 or 9 individual measurements. The corresponding error bars represent the 95% confidence interval in the mean of the independent measurements. The blue shaded region represents the range bounded by the lowest and highest outputs among the independent measurements at a given input level. The solid black curve was calculated by fitting a simple Hill function to the experimental measurements.
ActiviatedPromoterEquation.png
Experiment1 Characteristic1 Value1
Transfer Function Maximum Output 6.6 PoPS cell-1
Hill coefficient 1.6
Switch Point 1.5E-9 M 3OC6HSL, exogenous
Response time: <1 min
Input compatibility Strong response to 3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL
Weak response to C4HSL, C10HSL, C12HSL
Stability Genetic Stability
(Low/High Input) 		>92/>56 generations
Performance Stability
(Low/High Input) 		>92/>56 generations

Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002, E.Coli S30 Extract (data)
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC6HSL, C6HSL, C7HSL, C8HSL, C10HSL.

Pages needed for a device

  • Main page
  • Device Specification - experimental data and a model. Here is an example of a [http://openwetware.org/wiki/Registry_of_Standard_Biological_Models/Basic_Component_Models/Activated_Promoter model] for a biological device written in Cell ML. *User Comments and Usage Statistics
  • Availability information for physical DNA