Composite

Part:BBa_K1465222

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-05)

Carboxysome (shell-proteins (csoS14)) with translational GFP fusion (under T7 and without csoS2)


This part is the carboxysome from Halothiobacillus neapolitanus fused with GFP BBa_E0040 but without the shell-associated protein CsoS2, that is necessary for the correct folding of the microcompartment. The characterization of this part can be found at the page of the BioBrick BBa_K1465223 of the whole carboxysome including CsoS2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 704
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1853


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Categories
Parameters
None