Composite

Part:BBa_K3385059

Designed by: Daniel Bavnh�j   Group: iGEM20_DTU-Denmark   (2020-10-16)


T1G3R_SpmeA_glaA

About the part: Insert of glaA expression cassette for A. niger. It is made to be integrated in albA, which will lead to white/yellow conidia upon correct integration in the genome. The insert is inserted in pSB1C3 via USER cloning to create the integration plasmid for A. niger. The signal peptide was changed to SigP_pmeA using USER cloning and primer extension. The integration platform is made from part BBa_K3046030 pGIA2P1 designed by the DTU iGEM 2019 team.

Functionality: This signal peptide was investigated in Deeploc [1] to predict the localization of the protein which it is inserted upstream of.

Localization predicted by Deeploc.


Results: No insertion was successfully made, therefore the part needs to be investigated further.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5750
    Illegal EcoRI site found at 7178
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5781
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4012
    Illegal PstI site found at 6588
    Illegal PstI site found at 8322
    Illegal PstI site found at 8923
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5750
    Illegal EcoRI site found at 7178
    Illegal NheI site found at 4889
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4012
    Illegal PstI site found at 6588
    Illegal PstI site found at 8322
    Illegal PstI site found at 8923
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5750
    Illegal EcoRI site found at 7178
    Illegal BglII site found at 3867
    Illegal BglII site found at 5389
    Illegal BglII site found at 8420
    Illegal BamHI site found at 1729
    Illegal BamHI site found at 2157
    Illegal BamHI site found at 4847
    Illegal BamHI site found at 5649
    Illegal BamHI site found at 5775
    Illegal BamHI site found at 6326
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
    Illegal XhoI site found at 6186
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5750
    Illegal EcoRI site found at 7178
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5781
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4012
    Illegal PstI site found at 6588
    Illegal PstI site found at 8322
    Illegal PstI site found at 8923
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5750
    Illegal EcoRI site found at 7178
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5781
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4012
    Illegal PstI site found at 6588
    Illegal PstI site found at 8322
    Illegal PstI site found at 8923
    Illegal NgoMIV site found at 2566
    Illegal NgoMIV site found at 5107
    Illegal NgoMIV site found at 8310
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3395
    Illegal BsaI site found at 3533
    Illegal BsaI site found at 4222
    Illegal BsaI.rc site found at 4715
    Illegal SapI site found at 8811
    Illegal SapI site found at 9120


References:
[1] Jose Juan Almagro Armenteros, Casper Kaae Sønderby, Søren Kaae Sønderby, Henrik Nielsen, Ole Winther; DeepLoc: prediction of protein subcellular localization using deep learning, Bioinformatics, btx431

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