Composite

Part:BBa_K3046030

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-10-21)


pGIA2P1 insert

This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 2116
    Illegal EcoRI site found at 3552
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 2147
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 2954
    Illegal PstI site found at 4696
    Illegal PstI site found at 5297
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 2116
    Illegal EcoRI site found at 3552
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 2954
    Illegal PstI site found at 4696
    Illegal PstI site found at 5297
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 2116
    Illegal EcoRI site found at 3552
    Illegal BglII site found at 4794
    Illegal BamHI site found at 1729
    Illegal BamHI site found at 2141
    Illegal BamHI site found at 2692
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
    Illegal XhoI site found at 2552
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 2116
    Illegal EcoRI site found at 3552
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 2147
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 2954
    Illegal PstI site found at 4696
    Illegal PstI site found at 5297
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 2116
    Illegal EcoRI site found at 3552
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 2147
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 2954
    Illegal PstI site found at 4696
    Illegal PstI site found at 5297
    Illegal NgoMIV site found at 4684
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5185
    Illegal SapI site found at 5494


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