Composite

Part:BBa_K3385058

Designed by: Daniel Bavnh�j   Group: iGEM20_DTU-Denmark   (2020-10-16)


T1G3R_SfaeA_glaA

About the part: Insert of glaA expression cassette for Aspergillus niger. It is made to be integrated in albA, which will lead to white/yellow conidia upon correct integration in the genome. The insert is inserted in pSB1C3 via USER cloning to create the integration plasmid for A. niger. The signal peptide was changed to SigP_faeA using USER cloning and primer extension. The integration platform is made from part BBa_K3046030 pGIA2P1 designed by the DTU iGEM 2019 team.

Functionality: This signal peptide was investigated in Deeploc [1] to predict the localization of the protein which it is inserted upstream of.

Localization predicted by Deeploc.


Results: No insertion was successfully made, therefore the part needs to be investigated further.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5762
    Illegal EcoRI site found at 7190
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5793
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 3126
    Illegal PstI site found at 4024
    Illegal PstI site found at 6600
    Illegal PstI site found at 8334
    Illegal PstI site found at 8935
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5762
    Illegal EcoRI site found at 7190
    Illegal NheI site found at 4901
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 3126
    Illegal PstI site found at 4024
    Illegal PstI site found at 6600
    Illegal PstI site found at 8334
    Illegal PstI site found at 8935
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5762
    Illegal EcoRI site found at 7190
    Illegal BglII site found at 3879
    Illegal BglII site found at 5401
    Illegal BglII site found at 8432
    Illegal BamHI site found at 1729
    Illegal BamHI site found at 2157
    Illegal BamHI site found at 4859
    Illegal BamHI site found at 5661
    Illegal BamHI site found at 5787
    Illegal BamHI site found at 6338
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
    Illegal XhoI site found at 6198
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5762
    Illegal EcoRI site found at 7190
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5793
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 3126
    Illegal PstI site found at 4024
    Illegal PstI site found at 6600
    Illegal PstI site found at 8334
    Illegal PstI site found at 8935
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5762
    Illegal EcoRI site found at 7190
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5793
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 3126
    Illegal PstI site found at 4024
    Illegal PstI site found at 6600
    Illegal PstI site found at 8334
    Illegal PstI site found at 8935
    Illegal NgoMIV site found at 2566
    Illegal NgoMIV site found at 5119
    Illegal NgoMIV site found at 8322
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3407
    Illegal BsaI site found at 3545
    Illegal BsaI site found at 4234
    Illegal BsaI.rc site found at 4727
    Illegal SapI site found at 8823
    Illegal SapI site found at 9132


References:
[1] Jose Juan Almagro Armenteros, Casper Kaae Sønderby, Søren Kaae Sønderby, Henrik Nielsen, Ole Winther; DeepLoc: prediction of protein subcellular localization using deep learning, Bioinformatics, btx431

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