Part:BBa_K2258003

pCAT_AmilCP

It expresses AmilCP constitutively under pCAT promoter PCAT promoter (BBa_I14033) Composite Amil CP (BBa_K1357009)

Usage and Biology

This part is useful as constitutively expressed a reporter.

iGEM17_IISER-Pune-India: Expression of chromoproteins. The images above show E coli constitutively expressing RFP BBa_E1010 (red), tsPurple BBa_K1033906 (purple) and amilCP BBa_K592009 (blue).

Photometric Characterization

iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) with an absorption peak at 590nm.

iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) the absorbance at different optical densities are normalized with respect to the peak absorbance.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]