Composite

Part:BBa_K2201407

Designed by: Olga Schmidt   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-27)


Barnase composite part with amber codons


This part contains the RNase Ba (Barnase) (EC 3.1.27) under control of the inducible araBAD promotor (BBa_K808000). It codes for an 12 kDa microbial ribonuclease which cleaves single stranded RNA. Barnase is found in Gram-positive bacteria Bacillus amyloliquefaciens.

Usage and Biology

The coding sequence contains two amber codons at amino acid postitions Q4Am and D46Am. The sequence is inverted (reverse). This composite part can be used to design a possible negative selection plasmid. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961

The coding sequence of this composite part is available in reverse.


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Categories
Parameters
None