Composite

Part:BBa_K2082232

Designed by: Pascal Schmidt   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-08)


RFP under the control of an optimized lacZ promoter combined with Gal11P:cI(434)

RFP under the control of an optimized PlacZ combinded with the fusion protein Gal11P:cI(434)

This part is a designed positive control for the bacterial two-hybrid system. It contains the necessery reporter construct with RFP as the reporter. Moreover, the generator of the fusion protein Gal11P:cI(434) is coded on this part as well. Together with BBa_K2082226 it is a possible control for the bacterial two-hybrid system. Due to time issues this part could not characterized. For a characterized fucntional positive control of the bacterial two-hybrid system the parts BBa_K2082221 and BBa_K2082231 can be used.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 841
    Illegal NheI site found at 864
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 656
    Illegal AgeI site found at 768
    Illegal AgeI site found at 1417
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None