Device

Part:BBa_K2082010

Designed by: Mikail Sahin   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Nanobody: Example binding protein Part of Nanobody Library (BBa_K2082006) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 166
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This device consists of:

  • an Anderson promoter (BBa_J23106)
  • a codon optimized RBS (BBa_B0034)
  • a RNA polymerase omega subunit
  • a cMyc-linker
  • the first Nanobody constant region
  • the Nanobody variable region
  • the second Nanobody constant region
  • a modified thr terminator (BBa_B1006)
  • .

    This device shows an example binding protein after the scheme portrayed in figure 1. It is a single example Nanobody fusion which is the perfect positive control for your designed Nanobody library or other specific binders for colony PCRs, sequencings and more.

    To get further insight into the theoretical assembly take a look here.
    If you are planning to build your own Nanobody library or construct a specific binder, take a look at our hints




    Figure 1:General scheme of Nanobody construct with variable regions. A schematic view of how variable regions are embedded in the Nanobody construct.


    Visit our wiki for more information about our library and the opportunity of building your own library. If you are interested in other binding proteins, check out our Monobody parts for setting up a Monobody library.


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    Parameters
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