Part:BBa_K165018

SV40 nuclear localization sequence + ADH1 transcription terminator

Built in the Silver Lab [http://2008.igem.org/Team:BrownTwo/Implementation/syntf Biofusion]standard.

The SV40 nuclear localization sequence codes for a series of amino acids that tag a protein to be transported into the nucleus in eukaryotes.[http://www.ncbi.nlm.nih.gov/pubmed/6096007] It can be added to any coding region to localize its expression to the nucleus in yeast. This is useful for building synthetic transcription factors. In this part, it is ligated to the ADH1 transcription terminator for convenient cloning to the end of coding sequences.

To build a transcriptional repressor, ligate a DNA-binding domain to this NLS+terminaor. Add the resulting part downstream of Kozak+Sin3 Repression Domain

The LexA repressor has also been shown to work with the Sin3 repression domain and DNA-binding domain switched, in this order:

Kozak + LexA DBD + Sin3 + NLS + Term

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]