Intermediate
KSin3

Part:BBa_K165028

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-27)

Kozak + Sin3 Repression Domain

Built in the Silver Lab [http://2008.igem.org/Team:BrownTwo/Implementation/syntf Biofusion]standard.

This is the kozak sequence ligated to the Sin3 Repression domain. It can be used to build synthetic transcriptional repressing factors by ligating it to any DNA-binding domain, optionally a tandem set of fluorescent proteins to track expression, and to the SV40 NLS +ADH1 terminator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 946
    Illegal SapI.rc site found at 1539


[edit]
Categories
Parameters
None