Plasmid_Backbone

Part:pSB6C5

Designed by: Jonathan Babb   Group: iGEM10_MIT   (2010-10-26)

pBR322 Derived Chloramphenicol Backbone

Made by the iGEM 2010 MIT team


This part is under construction. It is the corrected version of K415049 with the extra reverse sequencing site removed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2335
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2341
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2335
    Illegal BamHI site found at 1479
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2335
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2335
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2350
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 1414


[edit]
Categories
Parameters
None