Plasmid_Backbone

Part:pSB6C5:Design

Designed by: Jonathan Babb   Group: iGEM10_MIT   (2010-10-26)


pBR322 Derived Chloramphenicol Backbone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2335
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2341
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2335
    Illegal BamHI site found at 1479
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2335
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2335
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2350
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 1414


Design Notes

To be entered


Source

To be entered

References