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Plasmid

Part:pSB1AT3-1

Designed by: Tom Knight   Group: iGEM_Registry   (2005-06-07)


pSB1AT3 with ccdB insert

Target plasmid for three antibiotic assembly. Tetracycline resistant. Contains an insert with a constitutive promoter and the ccdB coding region. The ccdB gene expression is lethal in normal cloning strains. The plasmid must be replicated in the special ccdB resistant strain DB3.1.

Usage and Biology

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 816
    Illegal EcoRI site found at 119
    Illegal XbaI site found at 134
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 119
    Illegal NheI site found at 2085
    Illegal SpeI site found at 817
    Illegal PstI site found at 831
    Illegal NotI site found at 125
    Illegal NotI site found at 824
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 119
    Illegal BamHI site found at 804
    Illegal BamHI site found at 2231
    Illegal XhoI site found at 1850
    Illegal XhoI site found at 3133
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 817
    Illegal EcoRI site found at 119
    Illegal XbaI site found at 134
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 119
    Illegal XbaI site found at 134
    Illegal SpeI site found at 817
    Illegal PstI site found at 831
    Illegal NgoMIV site found at 2257
    Illegal NgoMIV site found at 2625
    Illegal NgoMIV site found at 2785
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 254
    Illegal BsaI.rc site found at 3279

Functional Parameters

chassisDB3.1
copies100-300
insertccdB
mcsBiobrick
originpMB1
resistanceAT
[edit]
Categories
//classic/plasmid/uncategorized
Parameters
chassisDB3.1
copies100-300
insertccdB
mcsBiobrick
originpMB1
resistanceAT