Plasmid
Part:pSB1AT3-1:Design
Designed by: Tom Knight Group: iGEM05_Registry_Example (2005-06-07)
pSB1AT3 with ccdB insert
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 816
Illegal EcoRI site found at 119
Illegal XbaI site found at 134 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 119
Illegal NheI site found at 2085
Illegal SpeI site found at 817
Illegal PstI site found at 831
Illegal NotI site found at 125
Illegal NotI site found at 824 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 119
Illegal BamHI site found at 804
Illegal BamHI site found at 2231
Illegal XhoI site found at 1850
Illegal XhoI site found at 3133 - 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 817
Illegal EcoRI site found at 119
Illegal XbaI site found at 134 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 119
Illegal XbaI site found at 134
Illegal SpeI site found at 817
Illegal PstI site found at 831
Illegal NgoMIV site found at 2257
Illegal NgoMIV site found at 2625
Illegal NgoMIV site found at 2785 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 254
Illegal BsaI.rc site found at 3279
Design Notes
Source
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