Plasmid_Backbone
pSB1AK8

Part:pSB1AK8

Designed by: Monika Ciglic   Group: iGEM2006_Slovenia   (2006-10-14)

Eukaryotic terminator vector (formerly BBa_J52017)

This vector is BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

  • RS 03:20, 9 December 2009 (UTC): This plasmid backbone is not fully compliant with Assembly standard 10. It is missing a C in the BioBrick suffix. The NotI and PstI sites overlap as the sequence is currently specified in the Registry. It is not known whether this error is simply a user entry error. Or if the deletion is present in the physical DNA as well.
  • RS 19:58, 24 November 2008 (UTC): The original entry of this part included both a plasmid and plasmid backbone. It has been split into a plasmid backbone and part. The physical Registry distribution has been updated accordingly.

The original part is now pSB1AK8-BBa_P1010.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XhoI site found at 1272
    Illegal XhoI site found at 2298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3405
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3405
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2444
    Illegal EcoRI site found at 3405
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16


[edit]
Categories
//chassis/eukaryote
//plasmidbackbone/operation
Parameters
chassis
copies
mcsBioBrick
originpMB1
resistanceAK