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Plasmid

Part:pSB1AK3-1

Designed by: Tom Knight   Group: iGEM_Registry   (2005-06-06)


PSB1AK3 with ccdB insert

Target plasmid for three antibiotic assembly. The ccdB insert prevents growth of the unmodified plasmid in normal cloning strains, allowing selection of only correctly assembled recombinants.

Usage and Biology

pSB1AK3 plasmid with a ccdB insert and constitutive promoter. The expressed ccdB protein is lethal to normal E. coli cells. This plasmid can only be grown in resistant cell strains, such as DB3.1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 816
    Illegal EcoRI site found at 119
    Illegal XbaI site found at 134
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 119
    Illegal SpeI site found at 817
    Illegal PstI site found at 831
    Illegal NotI site found at 125
    Illegal NotI site found at 824
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 119
    Illegal BamHI site found at 804
    Illegal XhoI site found at 1850
    Illegal XhoI site found at 2876
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 817
    Illegal EcoRI site found at 119
    Illegal XbaI site found at 134
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 119
    Illegal XbaI site found at 134
    Illegal SpeI site found at 817
    Illegal PstI site found at 831
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 254
    Illegal BsaI.rc site found at 3022

Functional Parameters

chassisDB3.1
copies100-300
insertccdB
mcsBiobrick
originpMB1
resistanceAK
[edit]
Categories
//classic/plasmid/uncategorized
Parameters
chassisDB3.1
copies100-300
insertccdB
mcsBiobrick
originpMB1
resistanceAK