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Plasmid

Part:pSB1AK3-1:Design

Designed by: Tom Knight   Group: iGEM05_Registry_Example   (2005-06-06)


PSB1AK3 with ccdB insert


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 816
    Illegal EcoRI site found at 119
    Illegal XbaI site found at 134
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 119
    Illegal SpeI site found at 817
    Illegal PstI site found at 831
    Illegal NotI site found at 125
    Illegal NotI site found at 824
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 119
    Illegal BamHI site found at 804
    Illegal XhoI site found at 1850
    Illegal XhoI site found at 2876
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 817
    Illegal EcoRI site found at 119
    Illegal XbaI site found at 134
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 119
    Illegal XbaI site found at 134
    Illegal SpeI site found at 817
    Illegal PstI site found at 831
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 254
    Illegal BsaI.rc site found at 3022

Design Notes

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