Promoter (cinR and HSL regulated)
-- No description --
Usage and Biology
This "promoter" is the intergenic region from the CinRI locus. In Rhizobium leguminosarum, cinR and cinI are forward-pointing, adjacent, and separate transcriptional elements. There is a short (<200bp) intergenic region between cinR and cinI. The cinR/intergenic/cinI structure is conserved for the cinRI locus in Rhizobium etli, and for the cerRI locus in Rhodobacter sphaeroides. The intergenic region is thought to contain a terminator for cinR, a promoter and RBS for cinI, and a dna-binding domain for cinR and its homologs, though a conserved structure has not yet been identified. The BBa_R0077 RBS has been lopped off.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.