Part:BBa_K909009

cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana

UVR8 (UV resistance locus 8) is UV-B photoreceptor found in plants as a crucial mediator of plants response to UV-B response. In absence of UV-B the protein occurs as a dimer, which breaks upon UV-B irradiation.

Usage and Biology

Differently from other photoreceptors, UVR8 uses tryptophane residues present in the dimer interfase, rather than external chromophores. Energy from UV-B light is absorbed by these triptophanes and later used to break dimer cross-salt bridges resulting in the dissociation of the UVR8 monomers [http://www.sciencemag.org/content/335/6075/1492.long (Christie 2012)]. Monomeric UVR8 is then able to initiate downstream reactions that trigger the transcription of proteins needed for the UV-B caused stress response [http://www.cell.com/trends/plant-science/abstract/S1360-1385%2812%2900008-8 (Heijde 2012)].

We used UVR8 to create a novel UV-B switch in E.coli, by fusing it with TetR DNA binding domain (BBa_K909008). Additionally we inserted BamHI site after ATG codon for similar protein-protein fusions with this biobrick.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 560
  Illegal PstI site found at 1214
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 76
  Illegal PstI site found at 560
  Illegal PstI site found at 1214
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 560
  Illegal PstI site found at 1214
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 560
  Illegal PstI site found at 1214
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 91