Coding

Part:BBa_K909008

Designed by: Gintautas Vainorius   Group: iGEM12_ETH_Zurich   (2012-09-23)

tetR-DBD-UVR8 fusion protein

Tetracycline repressor DNA binding domain (BBa_K909007) fusion with the N-terminus of the truncated version of UVR8 (BBa_K909009).


Usage and Biology

TetR-DBD (1-127 amino acids of original TetR) unable to bind DNA and repress transcription from TetR responsive promoter Ptet due to the lacking dimerization domain. Nevertheless, fusions with dimerizing proteins restores TetR-DBD DNA binding activity.

UVR8 is a plant UV-B photoreceptor, which switches from dimeric to monomeric form in the presence of UV-B. By fusing UVR8 with DNA binding domain of TetR, we can regulate gene expression with UV-B light from Ptet (figure 1).


Figure 1. UV-B light induced derepression by TetR-DBD-UVR8]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 902
    Illegal PstI site found at 1556
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 418
    Illegal PstI site found at 902
    Illegal PstI site found at 1556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 382
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 902
    Illegal PstI site found at 1556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 902
    Illegal PstI site found at 1556
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 433


[edit]
Categories
//cds/membrane/receptor
Parameters
None