Coding

Part:BBa_K909001

Designed by: Isaak Mueller   Group: iGEM12_ETH_Zurich   (2012-09-24)

Phycocyanobilin synthesis operon

This operon contains the coding regions of heme oxigenase (ho1) BBa_I15008 and ferredoxin oxireductase (PcyA) BBa_I15009 with strong ribosomal binding site BBa_B0034 and under control of a medium constitutively active promoter BBa_J23108.

ho1 and PcyA are enzymes from Synechocystis that facilitate the synthesis of Phycocyanobilin from heme.

Phycocyanobilin functions as the chromophore for the red light sensitive protein Cph1 (and also for the fusion protein Cph8 BBa_I15010)

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1607
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None