Strong promoter, strong RBS combination for high expression levels of proteins
The part is composed of sub-parts J23100 and B0034.
This is a strong promoter, strong RBS combination for high expression levels of proteins. It has a consensus constitutive promoter and RBS sequence-produces strongest possible expression.
Use of this Part by Team Michigan '12
Team Michigan '12 used this part as their primary protein-generator. Our data suggests that we were successful in using it to express FimE and HBiF recombinases for our recombinase-based switch systems. See our results page for more information.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC