Generator

Part:BBa_K880005

Designed by: Josh Atkinson, Mike Ferguson, and Ben Parker   Group: iGEM12_Michigan   (2012-09-29)

Strong promoter, strong RBS combination for high expression levels of proteins

The part is composed of sub-parts J23100 and B0034.

This is a strong promoter, strong RBS combination for high expression levels of proteins. It has a consensus constitutive promoter and RBS sequence-produces strongest possible expression.

Use of this Part by Team Michigan '12

Team Michigan '12 used this part as their primary protein-generator. Our data suggests that we were successful in using it to express FimE and HBiF recombinases for our recombinase-based switch systems. See our results page for more information[1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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