Coding

Part:BBa_K848001

Designed by: Antoine Ribeiro   Group: iGEM12_Bordeaux   (2012-09-26)

RBS-LsrK

We added a strong RBS (BBa_B0034) to the LsrK protein (BBa_K091002) in order to make it a transcriptional unit ready to use. The LsrK protein phosphorylates AI-2 signal molecule. Phosphorylated AI-2 will prevent LsrR protein (BBa_K848000) from inhibiting pLsrA promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 573
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


Note: This part did not have a reference sequence. A reference sequence has since been added based on the part's documentation; an intermediate part using the following basic parts: BBa_B0034 - BBa_K091002 - iGEM HQ

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Categories
Parameters
None