Coding

Part:BBa_K848000

Designed by: Antoine Ribeiro   Group: iGEM12_Bordeaux   (2012-09-26)

RBS-LsrR

We added a strong RBS (BBa_B0034) to the LsrR repressor (BBa_K091001) in order to make it a transcriptional unit ready to use. The protein is a repressor of the pLsrA promoter; pAI-2 signal molecule binds to LsrR and inhibits its operation, therefore promoting transcription. LsrK protein (BBa_K848001), which phosphorylates AI-2 signal molecule, is needed for such an inhibition.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Note: This part did not have a reference sequence. A reference sequence has since been added based on the part's documentation; an intermediate part using the following basic parts: BBa_B0034 - BBa_K091001 - iGEM HQ

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Categories
Parameters
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