Coding
Part:BBa_K848000
Designed by: Antoine Ribeiro Group: iGEM12_Bordeaux (2012-09-26)
RBS-LsrR
We added a strong RBS (BBa_B0034) to the LsrR repressor (BBa_K091001) in order to make it a transcriptional unit ready to use. The protein is a repressor of the pLsrA promoter; pAI-2 signal molecule binds to LsrR and inhibits its operation, therefore promoting transcription. LsrK protein (BBa_K848001), which phosphorylates AI-2 signal molecule, is needed for such an inhibition.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Note: This part did not have a reference sequence. A reference sequence has since been added based on the part's documentation; an intermediate part using the following basic parts: BBa_B0034 - BBa_K091001 - iGEM HQ
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Categories
Parameters
None |