Luminesensor repressible SulA408 promoter
SulA promoter in 408 form.
SulA promoter belongs to the SOS regulon family. In E.coli it promotes the transcription of SulA protein. LexA protein in E.coli can bind to SulA promoter, inhibiting the transcription of the genes downstream.
However, with mutations in specific sites which LexA protein will bind to, SulA408 promoter will not be bound by LexA protein, but is compatible to LexA408 protein with corresponding mutations in DNA binding site. So a bio-orthogonal promoter is formed because the influence of endogenous LexA protein is excluded.
For example, the optimized Luminesensor with a LexA408 domain (BBa_K819005) constructed by Peking 2012 iGEM team can specifically bind to SulA408 promoter and therefore inhibit the gene expression (e.g. BBa_K819006 is a testing device containing SulA408 promoter constructed by Peking 2012 iGEM team)
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.
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