Medium cheZ generator
CheZ is a significant protein in E.coli chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.
CheZ was placed under a weak promoter BBa_J23113. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain.
Figure 1. E.coli was grown on semi-solid medium. (a) MG1655 strain with cheZ gene deleted; (b) CheZ gene under weak promoter BBa_J23112 (BBa_K819009); (c) CheZ gene under medium promoter BBa_J23113 (this part); (d)wild type MG1655 strain;
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.
|n/a||Medium cheZ generator|