Composite

Part:BBa_K784006

Designed by: Ilya Vainberg Slutskin   Group: iGEM12_Technion   (2012-09-13)

Theophylline riboswitch 12.1 + mCherry

This part is a direct fusion of the theophylline riboswitch (BBa_K784005) and the mCherry protein (BBa_J06504) which was amplified from BBa_J06702. The part was generated by Assembly pcr. This part was to be used in order to characterize the effect of theophylline concentration on the level of translation of the mCherry protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters: Austin_UTexas

BBa_K784006 parameters

Burden Imposed by this Part:

Burden Value: -0.4 ± 2.3%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.

[edit]
Categories
//function/reporter
//function/reporter/fluorescence
Parameters
abs --
biology
emission610
emit610
excitation587
excite587
lum --
proteinmCherry2
tagNone