Generator

Part:BBa_K778008

Designed by: Yuka Yamazaki   Group: iGEM12_UT-Tokyo   (2012-09-23)

FhlA-E363G generator (pLac-RBS-fhlA363-d.term)

plac-rbs-fhlA363-d.term

FhlA-E363G generator (pLac)

FhlA (Part:BBa K778001) is the transcriptional activator of hycAp (Part:BBa K778000). E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

fhlA-E363G has one point mutation (A to G, glutamic acid to glycine).

This BioBrick part can be used to overexpress FhlA-E363G with IPTG induction for enhanced hydrogen production and for more H2 production than FhlA(wild-type) generator.

UT Tokyo H2 1001+eg.png

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System] but the results in this wiki is older than this figure above.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2178
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1457
  • 1000
    COMPATIBLE WITH RFC[1000]


Safety.png

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Categories
Parameters
None