Coding
fhlA

Part:BBa_K778001

Designed by: Tomohito Minakuchi   Group: iGEM12_UT-Tokyo   (2012-09-22)

fhlA: a transcriptional activator for enhanced hydrogen production


FhlA is the transcriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

UT Tokyo H2 1001+cm.png

(The full construct in this experiment is Part:BBa K778007 and the modified fhlA(E363G) part is Part:BBa K778002.)

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] but the results in this wiki is older than this figure above.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1375
  • 1000
    COMPATIBLE WITH RFC[1000]


Safety.png

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Categories
Parameters
None