Part:BBa_K777103

flhDC operon under the control of constitutive promoter J23105

Fig. 1: Overview of BioBricks containing the flhDC operon downstream of Anderson promoters. (Activity according to Berkeley 2006)

The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes.

Here we used a set of 8 constitutive Anderson promoters from the 2006 Berkeley group to test how different levels of constitutive flhDC expression affect the motility of E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 650
  
• 1000
  COMPATIBLE WITH RFC[1000]