Part:BBa_K777005

Tar receptor under the control of constitutive promoter J23109

Fig. 1: Overview of BioBricks containing the Tar gene downstream of Anderson promoters. (Activity according to Berkeley 2006)

The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer.

Here, we used a set of 8 constitutive Anderson promoters from the 2006 Berkeley group to test how different levels of constitutive Tar expression affect the chemotaxis of E. coli (Fig. 1).

The transcriptional activity of the constructs K777001-K777008 was quantified via quantitative real-time PCR. The results of this experiment can be found in the experience section of the according Anderson promoters. Our data were compared to the activity of the promoters that can be found on the main page of Anderson promoters.
The respective raw dataset can be found in our Team-wiki [http://2012.igem.org/Team:Goettingen/Project/Computational_Data#Raw_Datasets here].

Usage and Biology

This part can be used for chemotaxis assays.

• Here is the complete sequence of this part as an ApE file.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1323
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 152
  

Fig. 2: Plasmid map of K777005 in pSB1C3