Ptms - a constitutive promoter in E. coli and B. subtilis.
Ptms is a constitutive promoter that can be recognized by SigA factor in Bacillus subtilis. Due to E. coli’s promiscuousness in recognizing transcription and translation signals, Ptms is also functional in E. coli. According to some experiments, Ptms shows quite low efficiency of transcription in B. subtilis. ( Moran et al., 1982).
For the characterization of Ptms, please refer to: BBa_K733009
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.
Moran, C., Lang, N., LeGrice, S., Lee, G., Stephens, M., Sonenshein, A., et al. (1982). Nucleotide sequences that signal the initiation of transcription and translation inBacillus subtilis..Molecular and General Genetics MGG,186, 339-346.