Generator

Part:BBa_K620002

Designed by: Caltech iGEM 2011   Group: iGEM11_Caltech   (2011-09-20)

LacI promoter + BisdA

Allows for inducible expression of the protein BisdA, one of a pair of proteins that can degrade bisphenol A (BPA). It is controlled by the lacI promoter, which can be induced by IPTG. It should be used in conjuction with K620003 to degrade BPA.

Usage and Biology

Plasmid map of K620002 in pSB4A5


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 216
    Illegal AgeI site found at 534
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 407
    Illegal BsaI site found at 443


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Categories
Parameters
None