Device

Part:BBa_K598014

Designed by: Xiaowei YAN, Tong MU   Group: iGEM11_pku_riboclamp   (2011-09-27)

pBAD+HH+CdIntron+E0040+b0015


This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.

It is the positive control of the theophylline inducing experiment. For further information, please see ,BBa_K598013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1408



Functional Parameters: Austin_UTexas

BBa_K598014 parameters

Burden Imposed by this Part:

Burden Value: -2.1 ± 2.4%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.

[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//plasmidbackbone/copynumber/high
Parameters
emission509nm
excitation470nm
n/apBAD+HH+CdIntron+E0040+b0015
resistancechloramphenicol