Part:BBa_K588001

ptrp

Tryptophan promoter and operator

Usage and Biology

ptrp is a promoter with the trp operator. It will be repressed by trpR (K588000) and LovTAP.

Note that the trp operator contains two SpeI sites which are critical for trpR binding. Thus, using UNAM 2010's idea, we cloned the part with an NheI site in the suffix instead of the SpeI site. When using this BioBrick, be sure to ligate it last and use NheI instead of SpeI.

This part is a clone of K360023

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 23
  Illegal SpeI site found at 31
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 23
  Illegal SpeI site found at 31
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 23
  Illegal SpeI site found at 31
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 23
  Illegal SpeI site found at 31
  
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters: Austin_UTexas

BBa_K588001 parameters

Burden Imposed by this Part:

Burden Value: 1.2 ± 5.6%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.