Composite

Part:BBa_K575014

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-08-11)

RhlR/PAI2 Inducible Promoter + RBS (B0030) + RFP

RhlR/PAI2 inducible promoter with RBS (B0030) and RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 637
    Illegal AgeI site found at 749
  • 1000
    COMPATIBLE WITH RFC[1000]




Functional Parameters: Austin_UTexas

BBa_K575014 parameters

Burden Imposed by this Part:

Burden Value: 7.9 ± 16.1%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.

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Categories
Parameters
n/aRhlR/PAI2 Inducible Promoter + RBS (B0030) + RFP