Part:BBa_K566004

CFP protein generator without transcription terminator

Protein generator of enhanced cyan fluorescent protein (ECFP) without transcription terminator. It may be negatively regulated by Mnt repressor (BBa_C0072). CFP coding sequence was optimized from part BBa_E0022, with preferential codon usage for improved expression in E. coli.

Usage and Biology

Is a monomeric protein generated on the basis of GFP-like protein from jellyfish Aequorea victoria . It possesses bright fluorescence with excitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1, which means how strongly a chemical species absorbs light at a given wavelength. It has low absortion and low quantum yield (40%). The fluorescence quantum yield gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed. Modified with a SsrA tail for rapid degradation of the protein and faster fall time for the emission.

Figure 1. Relative fluorescence of ECFP. Fluorescence excitation/emission maxima at 434 and 477 nm, respectively. Figure taken and modified from Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. Cell Science at a Glance 5:837-838 .

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 39
  Illegal XhoI site found at 1
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 107
  
• 1000
  COMPATIBLE WITH RFC[1000]