enhanced cyan fluorescent protein derived from A. victoria GFP
Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
ECUST_China 2019 Charaterization
In ECUST_China 2019 characterization，we constructed a plasmid containing pLac and CI protein, adding BBa_E0022 eCFP as the reporter gene. We attempted to verify the function of this plasmid via observing the eCFP fluorescence by the comparison between the negative control and induced cells.
Figure 1. Fluorescence intensity of eCFP
However, since the CI and eCFP was polycistron, both under the control of pLac, the expression of eCFP was disturbed by CI. The figure illustrated that as time went by, the expression of eCFP decreased, contrary to the prospective results. The characterization of eCFP in ECUST_China showed that BBa_E0022 was not suitable for the characterization of polycistron structure.
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.