Composite

Part:BBa_K553003

Designed by: Giulio Bernardinelli, Veronica Parisi, Francesca Cesaratto   Group: iGEM11_UNITS_TRIESTE   (2011-09-17)

constitutive production of LasR trans-activator

This part produces constitutively the P. aeruginosa LasR trans-activator.

The generated protein binds OC12 HLA and regulates positively PromLasB promoter

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
None