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Composite

Part:BBa_K539732

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-24)

kivd (alpha-ketoisovalerate decarboxylase) with native rbs(BBa_B0030) and terminator

This is the coding sequence of kivd from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination.Kivd plays a vital rule in the isobutanol biosynthesis pathway.
The rbs(BBa_B0030) and terminator(BBa_J61048)are included.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 599
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1676
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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