Intermediate

Part:BBa_K539643

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Taiwan   (2011-09-27)

a RBS (B0034)+ilvC with a RBS (B0034)+ilvD

Built in two strong expressing RBSs, ilvC and ilvD (Part:BBa_K539623 with Part:BBa_K539626). It catalyze 2-Acetolactate into 2-Ketoisovalerate in isobutanol biosynthesis pathway and could be applied in your interest part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 610
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 196
    Illegal BsaI site found at 3027
    Illegal BsaI site found at 3284
    Illegal BsaI.rc site found at 1015


[edit]
Categories
Parameters
n/aa RBS (B0034)+ilvC with a RBS (B0034)+ilvD