Intermediate

Part:BBa_K539625

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

a RBS (B0032)+ilvD

Built in weak expressing RBS (Part:BBa_B0032) and ilvD (Part:BBa_K539712). It catalyze 2,3-Dihydroxy-isovalerate into 2-Ketoisovalerate in isobutanol biosynthesis pathway and could be applied in your interest part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1740
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1526
    Illegal BsaI site found at 1783


[edit]
Categories
Parameters
n/aa RBS (B0032)+ilvD