Composite

Part:BBa_K539558

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Taiwan   (2011-09-27)

a RBS+lacI repressor+RNA thermometer+tetR+double terminator

Built in strong expressing RBS(part:BBa_B0034), LacI(Part:BBa_C0012), 37’celcius regulator RBS, tetR and Terminator(Part:BBa_K332033). It inhibit Plac in any temperature and Ptet contained circuit when the temperature hit 37’C or higher. It can be applied in your interest part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1930
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aa RBS+lacI repressor+RNA thermometer+tetR+double terminator