Reporter

Part:BBa_K516030

Designed by: Nicolo' Politi, Federica Sampietro, Davide Bianchini   Group: iGEM11_UNIPV-Pavia   (2011-09-08)

mRFP protein generator TERM+ RBS+(B0030)


Red fluorescent protein generator with BBa_B0030 high efficiency RBS (from Community Collection) and double terminator.
This construct is useful to measure the relative strength of promoters or the RBS efficiency in relationship with a promoter of interest.
To build a measurement device is only necessary assemble a standard biobrick promoter with this device (or another one of the collection: BBa_K516031 ; BBa_K516032)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 576
    Illegal AgeI site found at 688
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/amRFP protein generator TERM+ RBS+(B0030)